The Ti-plasmid and Plant Molecular Biology.
A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including A. tumefaciens, A. rhizogenes, A. rubi and A. vitis. Evolutionarily, the Ti plasmid is part of a family of plasmids carried by many species of Alphaproteobacteria.Members of this plasmid family are defined by the presence of a conserved DNA region known as the repABC gene cassette, which.
Plasmid DNA purification kit selection guide Our plasmid DNA purification kits help you obtain reliable, high-quality plasmid DNA in minutes. We provide a wide range of formats from mini- to gigapreps as well as options for high-throughput processing.
Plasmid Purification Kits in Molecular Biology Sigma-Adlrich provides kits for the isolation of high quality plasmid and Bacterial Artificial Chromosome (BAC) DNA. Suitable for work with large and small scale plasmid, endotoxin-free plasmid, and BAC DNA.
During plasmid purification, bacterial cells are lysed, freeing DNA and other cellular components from the cell wall. Cellular components are then removed, and the DNA-containing lysate is processed to further remove contaminants separate the plasmid DNA from the genomic DNA.
Purification of the Plasmid DNA All methods of lysing bacteria will yield plasmid solutions contaminated with chromosomal DNA and RNA. Centrifugation removes the vast majority of chromosomal DNA (it will form a pellet, while plasmid DNA remains soluble), and treatment with RNase will eliminate contaminating RNA.
Plasmid, in microbiology, an extrachromosomal genetic element that occurs in many bacterial strains. Plasmids are circular deoxyribonucleic acid (DNA) molecules that replicate independently of the bacterial chromosome. They are not essential for the bacterium but may confer a selective advantage.
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